Prior research suggests that spirituality is positively related to well-being. Nevertheless, within-person variability in spirituality has yet to be addressed. Do people experience greater spirituality on some days versus others? Does daily spirituality predict daily well-being? Do within-person relationships between spirituality and well-being vary as a function of trait spirituality? The authors examined such questions using a daily diary study with 87 participants who provided reports of their daily spirituality and well-being for a total of 1,239 days. They found that daily spirituality was positively related to meaning in life, self-esteem, and positive affect, and the link from daily spirituality to both self-esteem and positive affect was fully mediated by meaning in life. Moreover, within-person relationships between daily spirituality and self-esteem and meaning in life were stronger for people higher in trait spirituality. Lagged analyses found positive relationships between present day spirituality and next day’s meaning in life; there was no evidence for meaning in life as a predictor of the next day’s spirituality. When focusing on affect, for people higher in trait spirituality, greater negative affect (and lower positive affect) predicted greater spirituality the next day. These results provide new insights into how spirituality operates as a fluctuating experience in daily life.